From Germs to Mammals in Aqua
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Genetics of Aquatic Organisms 2023, Vol 7, Num, 4     (Pages: GA715)

Effective Technology of Carp (Cyprinus carpio) Sperm Cryopreservation for Use in a Large-Scale Cryobank

Olga Dokina 1 ,Konstantin Kovalev 1 ,Natalia Pronina 1

1 Branch for the freshwater fisheries (VNIIPRKh) of the Federal State Budget Scientific Institution “Russian Federal Research Institute of Fisheries and oceanography”, v.Rybnoe 40A, Moscow region, Dmitrov city district, Russia DOI : 10.4194/GA715 Viewed : 1646 - Downloaded : 275 Preliminarily developed technology of cyprinids sperm cryopreservation was improved by optimization of protective medium composition. Effectiveness of this medium was confirmed by examination of trial media as well as by freezing sperm samples for storage in cryobank. Protective medium for cyprinid fishes sperm is aqueous solution consisting of 0.1% sucrose, 0.35% sodium chloride, 20-24% methanol and 3-6% ethylene glycol. When used, this medium stably ensured cryoprotection of sperm cells of different quality and conservation of its fertility at the level of native sperm. Results of evaluation of quality of cryopreserved cyprinid sperm samples before putting them in a low-temperature gene bank of VNIIPRKh are presented. Keywords : Cryoprotectant Cryopreserved sperm Cyprinid fishes