From Germs to Mammals in Aqua
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Genetics of Aquatic Organisms 2022, Vol 6, Num, 2     (Pages: GA496)

The Effect of Benzo[a]pyrene on Xenobiotic Metabolizing Cytochrome P4501A (CYP1A) Expression in Spotted Scat, Scatophagus argus (Linnaeus, 1766)

Ahmad Shadi 1 ,Ahmad Ghasemi 2

1 Persian Gulf University, Faculty of Nano and Bio Science and Technology, Department of Biological Science and Technology, Bushehr, Iran 7516913817
2 Persian Gulf Research Institute, Bushehr, Iran 7516913817
DOI : 10.4194/GA496 Viewed : 789 - Downloaded : 388 Low molecular weight Polycyclic Aromatic Hydrocarbons (PAHs) are widely concerned due to their significant health risks such as carcinogenicity, reproductive- and endocrine-disrupting effects, immunotoxicity and neurotoxicity on human and marine animals. The induction pattern of xenobiotic metabolizing cytochrome P4501A (CYP1A1) gene was studied on 150 spotted scat Scatophagus argus (weight 32±3 g) captured from Northern Persian Gulf and exposed to 0, 5, 10, 15, 20 mg kg-1 of benzo[a]pyrene in 100L aquarium. The induction of CYP1A1 was quantified using reverse-transcription real-time PCR from treated fish liver samples. The results revealed that the expression of CYP1A1 gene in S. argus liver is time-dependent and directly correlated to the concentration of B[a] P, therefore the study provides the basis for further use of CYP1A1 of this commercially ad ecologically important species as a biomarker. Keywords : Biomarkers Scatophagus argus PAHs cDNA cloning Xenobiotics