From Germs to Mammals in Aqua
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Genetics of Aquatic Organisms 2022, Vol 6, Num, 2     (Pages: GA480)
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Isolation and Expression Analysis of cAMP-Dependent Protein Kinase and Adenylyl Cyclase-Associated Protein 1-like cDNAs in the Giant Tiger Shrimp Penaeus monodon

Sirawut Klinbunga 1 ,Kanchana Sittikankaew 2 ,Sirikan Prasertlux 1 ,Sirithorn Janpoom 1 ,Puttawan Rongmung 1 ,Wanwipa Ittarat 1 ,Phimsucha Boonpimpapha 1 ,Bavornlak Khamnamtong 1

1 National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Aquatic Molecular Genetics and Biotechnology Research Team, 113 Paholyothin Road, Khlong Nueng, Khlong Luang, Pathum Thani 12120, Thailand
2 National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Microarray Research Team, 113 Paholyothin Road, Khlong Nueng, Khlong Luang, Pathum Thani 12120, Thailand DOI : 10.4194/GA480 Viewed : 1003 - Downloaded : 801 Characterization of genes exhibiting differential expression profiles during ovarian development is important for understanding reproductive maturation of the giant tiger shrimp (Penaeus monodon). Here, the partial cDNAs of P. monodon cAMP-dependent protein kinase, catalytic subunit 1 (PmPkaC1) and adenylyl cyclase-associated protein 1-like (PmCap1-l) were studied. They were more preferentially expressed in ovaries than testes of cultured juveniles and wild broodstock. PmPkaC1 mRNA in ovaries of non-ablated broodstock was significantly increased during vitellogenesis (P<0.05). However, unilateral eyestalk ablation (the removal of one eyestalk) resulted in a significant reduction of its expression (P<0.05). PmCap1-l was not differentially expressed during ovarian development in wild non-ablated broodstock. It was up-regulated in mature ovaries following eyestalk ablation (P<0.05). The PmCap1-l transcript in each ovarian stage of the former was significantly lower than that of the latter (P<0.05). The levels of ovarian PmPkaC1 and vitellogenin 1 (PmVtg1) treated in vitro with 17-20β-DHP (0.1, 1.0 and 10.0 μg/ml for 24 h) was not significantly different from the control (P>0.05). Nevertheless, the expression of PmCap1-l was increased in ovaries treated with 0.1 and 10 μg/ml 17-20β-DHP at 24 hours post treatment (hpt, P<0.05). Keywords : Penaeus monodon Eyestalk ablation Gene expression 17α,20β-dihydroxyprogesterone