Genetics of Aquatic Organisms
2022, Vol 6, Num, 1 (Pages: GA433)
Phylogenetic of Marble Goby (Oxyeleotris marmorata Blkr.) in South Sumatra Based on Cytochrome C Oxidase Subunit I (COI) Gene
Mochamad Syaifudin 1 ,Dade Jubaedah 1 ,Ferdinand Hukama Taqwa 1 ,Ria Octaviani 1
1 Universitas Sriwijaya, Faculty of Agriculture, Program Study of Aquaculture, Jl. Palembang-Prabumulih Km 32, Indralaya, Ogan Ilir 30862, South Sumatra, Indonesia
DOI :
10.4194/GA433
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Marble goby (Oxyeleotris marmorata) in Indonesia, spread across Sumatra,
Kalimantan and Papua. The purpose of this study was to utilize a sequence of
mitochondrial DNA Cytochrome Oxidase Subunit I gene, to analyze the phylogenetic
tree and genetic distance between cultured and captured populations. This research
was conducted on March-August 2020. The methods used in barcoding species were
DNA isolation, amplification using PCR (Polymerase Chain Reaction) and sequencing of
the COI mtDNA gene. The domesticated samples (n=3) were collected from Gandus
Fish Seed Center (GFSC), while the wild samples (n=3) were captured from the Musi
River in Beruge Village, Babat Toman District, both in Musi Banyuasin Regency. The
sequenced COI mtDNA gene fragments were obtained from the PCR method. Three
samples performed good PCR results, while the other three didn`t amplify properly.
After the editing process, the COI gene sequencing produced a nucleotide length of
610 bp. Based on the BLAST (Basic Local Alignment Search Tool), domesticated marble
goby (OMD2, OMD3) was in the same cluster with marble goby from the Musi River
(OMS3). The genetic distance indicated that two specimens of domesticated marble
goby were 100% identical, while the wild (OMS3) indicated a genetic distance of
0.0066 to domesticated species.
Keywords :
Cytochrome C Oxidase Subunit I gene, O. marmorata, Domesticated, PCR, South Sumatra