From Germs to Mammals in Aqua
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Genetics of Aquatic Organisms 2019, Vol 3, Num, 2     (Pages: 37-45)

Microsatellite DNA Polymorphism of Channa marulius Inhabiting River Jhelum

Uzma Hussain 1 ,Khalid Abbas 1 ,Tanveer Ahmed 1-2 ,Iram Qadeer 1-3

1 University of Agriculture, Department of Zoology, Wildlife and Fisheries, Faisalabad, Punjab, Pakistan
2 Khwaja Fareed University of Engineering and Technology, Department of Chemistry, Rahim Yar Khan, Pakistan
3 Government Sadiq College Women University, Bahawalpur, Punjab, Pakistan
DOI : 10.4194/2459-1831-v3_2_01 Viewed : 1466 - Downloaded : 1596 Wild populations of C. marulius have undergone a steady decline due to habitat deterioration, overexploitation and aquatic pollution. Genetic polymorphism by using microsatellite DNA markers was analyzed in natural populations of C. marulius. Samples were collected from different sites of river Jhelum viz. Trimu Headworks, Rasul Barrage, Mangla Dam, Jhelum Bridge, and Marala Dhand. A total of five microsatellite loci (CA05, CA07, CA08, CA09, and CA10) were used to evaluate genetic polymorphism. The overall populations of C. marulius showed a moderate level of heterozygosity. The mean observed heterozygosity (Ho) at all the five loci was 0.43 whereas expected heterozygosity (He) was 0.54. The pairwise population genetic differentiation (FST) ranged in between 0.000001 to 0.231955. Analysis of molecular variance (AMOVA) revealed that majority of variations among populations than within populations 62.05% and 33.03%, respectively. Significant deviation (P<0.05) from Hardy-Weinberg equilibrium was seen in all the populations with clear deficit heterozygosity suggesting inbreeding. A UPGMA tree based on Nei`s genetic distance matrix revealed that C. marulius populations were divided into two major clusters. Principle component analysis showed same clustering pattern. The present study about genetic structure of C. marulius populations will be fruitful for effective management and conservation of fisheries resources. Keywords : Wild fish, Genetic Structure, Genetic differentiation, Inbreeding, SSR Markers