From Germs to Mammals in Aqua
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Genetics of Aquatic Organisms 2019, Vol 3, Num, 1     (Pages: 31-36)

The Study of Morphometric and Molecular Paradiplozoon chazarikum Parasite in Caspian Sea the Rutilus frisii kutum

Rahanandeh Mohamamad 1 ,Rahanandeh Mahsa 2

1 Guilan Agricultural and Natural Resources Researc and Education Center Agricultural Research, Education and Extension Organization (AREEO), Rasht, Iran
2 Genetic Department of Islamic Azad University Tonekabon Branch, Tonekabon, Iran
DOI : 10.4194/2459-1831-v3_1_05 Viewed : 2143 - Downloaded : 1978 Different species of monognegens cause different damage to the gills of different fish, one of which is Paradiplozoon chazarikum parasite in the Rutilus frisii kutum. The parasites were identified by isolating the gills below the stereo microscope. After providing wet slurry, clarifying and monteting them, under the optical microscope, morphological and morphometric indices were evaluated. To investigate the molecular Paradiplozoon chazaricum parasite from two upstream Ribosomal primers, the upstream F '5-GTCGTCGATTGGTTTGTT GTC-3 and downstream R' 5- GAGTTGCAGTATAAGCTAGGCTG-3, for DNA amplication with PCR. As a result, the morphometric study of the parasite using a valid parasitological key as well as the sequence obtained from Paradiplozoon chazaricum parasite was compared with the sequence recorded in the gene bank. Finally, the parasite was identified.` Keywords : Paradiplozoon, Rutilus frisii kutum, Morphometric, Molecular