From Germs to Mammals in Aqua
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Genetics of Aquatic Organisms 2019, Vol 3, Num, 1     (Pages: 09-16)

Comparative Study of Metabolic Gene Regulation with Seasonal Fluctuation of Temperature in Rainbow Trout (Onchorynchus mykiss) Under Captive Conditions

Mohammad Iqbal Mir 1 ,Uzma Siddiqui 1 ,Rabindar Singh Patiyal 1

1 ICAR- Directorate of Coldwater Fisheries Research, Division of Fish Genetics & Breeding, Bhimtal-Uttarakhand India DOI : 10.4194/2459-1831-v3_1_02 Viewed : 2095 - Downloaded : 1835 The temperature is most important abiotic factor which influences growth, reproduction, survival of fishes. The nutritional uptake its utilization and conversion by metabolic pathway enzymes are highly dependent on external water temperature. The present study was carried out for comparative analysis of expression of metabolic genes in Winter and Summer. The blood glucose estimation was highest in February 86.175 mg/dl >100C and lowest in January 83.9 mg/dl >40C during Winter period, whereas in Summer, highest was estimated in June 105.32mg/dl >17.80C and lowest was in July 103.4 mg/dl >210C. The triglyceride levels estimated highest in February 396.74mg/dl and least in January 382mg/dl where as in Summer highest was in June 481.92mg/dl and lowest in July 471.77. It was observed that mRNA transcript levels of GK and FBpase were extremely low and GLUT2 expression level was maintained, due to Q10 effect at temperature ≤40C but at 8-100C although GK gene expression was very low but FBpase and GLUT2 were maintained slightly higher. The effect of temperature on metabolic gene expression will be very important for understanding the differential seasonal growth pattern in fishes. Keywords : Metabolic pathways, Glucose, Triglycerides, Glucokinase GK