From Germs to Mammals in Aqua
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Genetics of Aquatic Organisms 2019, Vol 3, Num, 1     (Pages: 01-07)

Triploid Black Sea Trout (Salmo labrax Pallas, 1814) Induced by Heat Shock and Evaluation of Triploidy with Different Techniques

Eyüp Cakmak 1 ,Ekrem Cem Cankiriligil 1 ,Zehra Duygu Düzgünes 2 ,Osman Tolga Ozel 1 ,Oguzhan Eroglu 3 ,Sirin Firidin 2

1 Central Fisheries Research Institute, Department of Aquaculture, Vali Adil Yazar cd. No:14 Yomra/Trabzon Turkey
2 Central Fisheries Research Institute, Department of Breeding and Genetics, Vali Adil Yazar cd. No:14 Yomra/Trabzon Turkey
3 Directorate of Provincial Agriculture and Forestry, 38090, Kocasinan/Kayseri, Turkey
DOI : 10.4194/2459-1831-v3_1_01 Viewed : 6031 - Downloaded : 3435 Triploid fish production has become important in the aquaculture sector, especially in the culturing and breeding of the big sized Salmonid species. Black Sea trout (Salmo labrax) is one of the promising Salmonid species for these purposes considering increased demand for this species in the aquaculture sector. In this research, triploid Black Sea trout were treated with heat shocks during reproduction and triploidy of obtained trouts were identified with different techniques. For this reason, heat shocks were applied at 26.5°C, 28°C and 36°C temperatures for 10 minutes during 30th minute of fertilization and triploidy was determined with fragment analysis, flow cytometry and classic hematological methods. Results indicate that all three temperatures were suitable for the successful reproduction of Black Sea trout. However, 26.5°C gives better results considering both the triploidy success and survival rates. Besides, all three determination techniques especially erythrocyte size measurements and flow cytometry can be used in evaluating of triploidy in Black Sea trout with precise results. Keywords : Salmo labrax, Flow cytometry, Erythrocyte, Trout allele, Salmonidae