From Germs to Mammals in Aqua
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Genetics of Aquatic Organisms 2023, Vol 7, Num, 4     (Pages: GA729)

Impact of Curcumin Supplementation on Sperm Characteristics and Gene Expression in Fish Sperm Freezing Medium

Zafer Doğu 1 ,Faruk Aral 2 ,Erdinç Şahinöz 1 ,Ebru Temiz 3 ,İsmail Koyuncu 3

1 Department of Fisheries and Aquaculture, Bozova Vocational High School, Harran University, Bozova 63850, Türkiye.
2 Çarşı PTT PK 138, Konya 42010, Turkey.
3 Department of Medical Biochemistry, Faculty of Medicine, Harran University, Sanliurfa 63050, Türkiye
DOI : 10.4194/GA729 Viewed : 206 - Downloaded : 159 This study aimed to determine whether curcumin addition in freezing media affected the ability of trout semen to resist cryodamage. Fish semen was collected and cryopreserved in a curcumin-supplemented freezing medium at 20 μM. Sperm motility parameters, and nuclear factor 2 gene expressions (NRF-2 and Keap-1) were assessed following freezing and thawing. Adding 20 μM curcumin to the sperm extender increased the rate and duration of motility, as well as the percentage of live sperm at 277 mOsm with a 1:3 dilution. However, the sperm quality decreased at 135 and 365 mOsm with 1:2, 1:3, and 1:4 dilutions. Following cryopreservation, curcumin-supplemented frozen-thawed semen exhibited significantly elevated levels of NRF-2 and Keap-1 gene expressions. The findings demonstrated that adding curcumin to freezing media protected fish and motility parameters against damage induced by the freeze-thawing process. Keywords : Trout Curcumin Sperm cryopreservation NRF-2 Keap-1