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¹ Hasanuddin University, Study Program of Marine and Coastal Aquaculture, Faculty of Vocational Studies, Makassar, 90245, Indonesia
² IPB University, Department of Aquaculture, Faculty of Fisheries and Marine Science, Bogor, 16128, Indonesia DOI : 10.4194/GA945 Viewed : 25 - Downloaded : 17 Understanding how feeding regimes regulate igf-1 is crucial for improving growth and carbohydrate use in giant gourami. This study evaluated igf-1 expression across several tissues its response to different diets and glucose tolerance tests. Juvenile fish (51.99±7.4 g) were fed commercial feed or giant taro leaves (Alocasia macrorrhizos) for 8 weeks in controlled aquaria (60×40×40 cm, 10 fish/tank). Growth performance was higher with commercial feed, showing greater specific growth rate (1.77 vs. 0.67 %/day). After 48 h fasting, liver, intestine, pancreas, muscle, brain, spleen, and heart samples were collected. The glucose tolerance test consisted by four injection treatments: saline, glucose (1 g kg⁻¹ body weight), double saline, and insulin+glucose (2 IU 100 g⁻¹ body weight and 1 g kg⁻¹ glucose). Liver igf-1 expression was quantified at 0, 6, and 18 h post-injection using qPCR. The liver igf-1 peaked among tissues but declined within 6 h after glucose or insulin+glucose injection (P<0.05). Feeding regime affected igf-1 only in the liver, with different feeds enhancing its response in the glucose tolerance test. These findings indicate hepatic igf-1 regulates carbohydrate metabolism in giant gourami through glucose utilization and insulin responsiveness. Keywords : Alocasia macrorrhizos Carbohydrate Gene expression igf-1 Metabolism