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¹ Laboratory of Genetics and Breeding, Faculty of Biology, Universitas Gadjah Mada, Jl. Tehnika Selatan, Sekip Utara, Yogyakarta 55281, Indonesia
² Laboratory of Animal Structure and Development, Faculty of Biology, Universitas Gadjah Mada, Jl. Tehnika Selatan, Sekip Utara, Yogyakarta 55281, Indonesia DOI : 10.4194/GA905 Viewed : 35 - Downloaded : 29 Batissa violacea is easily found in Southeast Sulawesi, where it is known locally as Pokea. The declining population of B. violacea in Southeast Sulawesi due to limited systematic studies underscores the need for conservation strategies. This study aimed to identify the species and evaluate the intraspecific genetic variation and genetic population structure of B. violacea from the rivers of Southeast Sulawesi based on PCR-amplified COI mitochondrial gene sequences. The results revealed that all samples belong to the B. violacea species according to both morphological and molecular analyses. Genetic analysis revealed low genetic diversity, with five haplotypes and four variable sites detected across populations. AMOVA results indicated that the primary source of genetic variation occurred within populations. No clear genetic separation was observed, as shared haplotypes suggest ongoing gene flow among populations. However, low diversity may reflect human impacts such as overfishing and habitat degradation, which contribute to genetic decline. These findings highlight a potentially vulnerable genetic structure. Ensuring habitat protection, sustainable practices, and enhancement of gene flow are crucial for conservation management. Overall, this study provides a valuable basis for conservation planning and emphasizes the importance of sustainable management of B. violacea in its natural habitat. Keywords : Batissa violacea COI DNA Barcoding Genetic variation Population structure