From Germs to Mammals in Aqua
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Genetics of Aquatic Organisms 2017, Vol 1, Num, 2     (Pages: 61-69)

Analysis of Microsatellite Data for Population Genetics of Aquatic Organisms

Rafet Cagri Ozturk 1 ,Ilhan Altinok 1

1 Karadeniz Technical University, Surmene Faculty of Marine Sciences, Department of Fisheries Technology Engineering, 61530 Surmene, Trabzon, Turkey DOI : 10.4194/2459-1831-v1_2_04 Viewed : 8892 - Downloaded : 12533 Genetic analysis is vital for conservation biology, stock management and for understanding evolutionary processes at a population level. Microsatellites are one of the most popular genetic markers in molecular ecology and population genetics, conservation and wildlife management. Microsatellites or simple sequence repeats are distributed throughout the genome of eukaryotes and prokaryotes and found in both coding and non-coding nuclear and organellar DNA. Non-coding microsatellites are ideal genetic markers to estimate population structure, gene flow, genetic diversity, genetic distance in wild population and to assess level of inbreeding, parentage analysis and to map quantitative traits in the domesticated and cultivated species. To study microsatellite loci, following workflow has to be completed. Isolation of DNA from small nonlethal tissue (fin clip) sample, amplification of microsatellite regions with multiplex PCR using fluorescent dye labelled primers, making of electropherogram of fragments with analyzer and analysis of the data with the population genetic software packages which enable to check data errors, calculate basic population parameters and sophisticated analyses. In this review, we described features and functionalities of programs useful for microsatellite data analysis. Keywords : Population genetic, genetic programs, error-checking, genetic parameters