From Germs to Mammals in Aqua
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Genetics of Aquatic Organisms 2017, Vol 1, Num, 2     (Pages: 57-60)

Optimizing DNA Extraction Method for Archived Otoliths

Sirin Firidin 1 ,Oguzhan Eroglu 1 ,Ilyas Kutlu 1

1 Central Fisheries Research Institute, Trabzon DOI : 10.4194/2459-1831-v1_2_03 Viewed : 785 - Downloaded : 402 The DNA from the archive collections is very important for fisheries biologists, administrators, systematics and conservation biologists. Archival tissues allow access to the genetic information required to resolve the link between past and present. However, effective protocols for obtaining DNA from otolith and bone are still insufficient, time consuming and inefficient.
The aim of this study was to develop an extraction protocol for otholits samples based on NucleoSpin TissueXS, Macherey-Nagel commercial kit and perform a quantitative comparison with the extraction protocol based on EDTA-SDS. Keywords : Archived otoliths, DNA extraction, EDTA-SDS