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Genetics of Aquatic Organisms 2018, Vol 2, Num, 2     (Pages: 53-60)

Genetic Diversity for Anchovy Populations (Engraulis encrasicolus L.) in the Azov, Marmara and Black Sea

Zehra Duygu Düzgüneş 1 ,Oğuzhan Eroğlu 2 ,Şirin Firidin 1 ,Eyüp Çakmak 1

1 Central Fisheries Research Institute, 61250, Trabzon, Turk
2 Directorate of Provincial Agriculture and Forestry, 38090, Kocasinan, Kayseri, Turkey
DOI : 10.4194/2459-1831-v2_2_05 Viewed : 100 - Downloaded : 73 In this study, the structure of the anchovy population in the Azov, Marmara and Black Sea was determined by restriction fragment length polymorphism (RFLP). Sampling was carried out at six stations represented the Azov, Marmara and Black Sea populations in 2011. Total DNA of the sample was taken each station and the mtDNA D-loop region within total DNA was amplified by PCR. Amplified mtDNA D-loop region by PCR (polymerase chain reaction) was cut by10 different enzymes (Hpa II, Hinf I, Hae III, Alu I, Ava II, Nci I, RsaI, TaqI, Tru91, Hsp9211) and morphs were determined by the type of the particle profiles for each population. mtDNA D-loop haplotypes were determined by combining this morphs. As a result, a total of 64 haplotypes belong to populations that were sampled from six stations (Azov Sea, Black Sea; Georgia, Abkhazia, Trabzon-Turkey winter, Trabzon-Turkey summer and Marmara Sea) have been identified. Fst values among populations were calculated by the analysis of molecular variance and the Marmara Sea populations clearly separated from other populations. Also it was seen that Trabzon-summer samplings were sharing haplotypes of all other populations. No evidence have found for recent population bottleneck of the population studies. Keywords : Anchovy, Engraulis encrasicolus, Population genetics, RFLP, mtDNA